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Microcomputer-controlled buffer gradient generator for ion-exchange chromatography  

作  者:A.M.C. Davies;E.H.A. Prescott;R. Stansfield

机构地区:Agricultural Research Council's Food Research Institute, Colney Lane, Norwich Great Britain ;

出  处:Journal of Chromatography A1979,171(000),117-133,17

语  种:外文

出 版 国:荷兰

摘  要:A microcomputer-based system has been developed for switching between several buffer solutions to produce a mixture with independent elution gradients for two ions. The system has been used as a gradient programmer for the separation of amino acids by ion-exchange chromatography and the computer also supervises the automatic facilities of an amino acid analyser. In normal operation there is no monitoring of the concentrations produce but results obtained with the system demonstrate that the performance is reproducible and a good approximation to the specified gradient is obtained. Advantages of the system are that it gives the analyser a high separating power and it is versatile and easy to use.

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